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. 2020 Jan 8;13:185–197. doi: 10.2147/OTT.S229577

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© 2020 Huang et al.

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Suppression of RCC cell migration and invasion ability by UBE4B silencing. (A, B) UBE4B knockdown using specific siRNAs inhibited the migration ability of ACHN (A) and SKRC39 (B) cells in a Transwell migration assay. (C, D) UBE4B silencing using specific siRNAs remarkably attenuated the invasion ability of ACHN (C) and SKRC39 (D) cells in a Matrigel invasion assay. Representative images are shown on the left (×200 magnification) with quantification of the numbers of cells in ten randomly-selected fields shown on the right. Data was shown as mean ± SD of three independent experiments. P-values were calculated using the Student’s t-test; **P <0.01, ***P <0.001, versus cells transfected with siNC. siNC represents negative control small interfering RNAs.