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. 2020 Jan 9;6(1):e03115. doi: 10.1016/j.heliyon.2019.e03115

Figure 1.

Figure 1

TRIM34 localized to both the mitochondria and cytosol. (A) HEK293T cells were transfected with the pEGFP-N3-TRIM34 vectors for 24 h. Then the cells were fixed, stained for nuclei using DAPI (blue) and analyzed by confocal microscope. Scale bar, 10 μm. (B) HEK293T cells were transfected with pEGFP-N3-TRIM34 or pEGFP-N3 vectors (control) for 24 h, then the mitochondria were stained with MitoTracker Deep Red and the nuclei were stained with DAPI (blue). Images were visualized at × 63 oil immersion objectives by confocal microscope. The scale bar represented 10 μm. The localization of TRIM34-EGFP to the mitochondria was marked with arrows. Representative results were presented, two additional experiments yielded similar results. (C) The Pearson's correlation coefficients were calculated by JACoP analysis plugin of ImageJ 1.50i. The scale bar represented 5 μm. (D) HEK293T cells were transfected with 5′FLAG-pcDNA3.1 (vector), 5′FLAG-pcDNA3.1-TRIM34 (TRIM34) or subjected to mock transfection for 24 h. The mitochondria fraction was prepared and the expression of TRIM34 was examined by western blot analysis. Representative results were presented and two additional experiments yielded similar results. (E) HEK293T cells were cotransfected with LC3B-EGFP/TRIM34-DsRed1 or LC3B-EGFP/TRIM22-DsRed1 for 48 h. Images were visualized at × 63 oil immersion objectives using confocal microscope. The scale bar represented 5 μm. Representative results were presented and two additional experiments yielded similar results.