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. 2020 Jan 9;6(1):e03115. doi: 10.1016/j.heliyon.2019.e03115

Figure 2.

Figure 2

Effects of TRIM34 on the cell viability and cell cycle. (A) HEK293T cells were transfected with 5′FLAG-pcDNA3.1-TRIM34 or control vectors for 24 h, and then subjected to the CCK8 assay. The cell viability was detected by absorbance which was measured at 450 nm. Results were obtained from at least three independent experiments, and data were showed as mean ± SD. (B) HEK293T cells were transfected with 5′FLAG-pcDNA3.1- TRIM34 or 5′FLAG-pcDNA3.1 vectors for 24 h. The cells were then harvested and stained with propidium iodide. The cell cycle distribution was analyzed by flow cytometry. Images were representative of three independent tests. (C) The cell cycle distribution was quantitative analyzed. Each bar represented means ± SD from three independent experiments.