Figure 2.

Studies of AGuIX® NPs on gliosarcoma and brain melanoma metastases rodent cancer models. A. MRI and Gd quantification in 9L-ESRF-bearing rats. Left panel: the T₁-weighted MR images were acquired before and 1, 4, 7 and 24 h after iv injection of 1 ml of AGuIX® NPs ([Gd³⁺] = 100 mM) over three rats 14 days after tumor implantation. The pictures show an enhancement of tumor-T₁ contrast due to the AGuIX® NPs, until 24 h p.i.. Right panel: Gd concentrations issued from T₁ maps as a function of time elapsed after iv injection (n = 3) in three regions of interest. The results were expressed as the means ± standard deviation. B. Survival curve comparison obtained for 9LGS-bearing rats without treatment (yellow curve, n = 5), only treated by microbeam RT (MRT) (green curve, n = 7), and treated by MRT 1 h (grey curve, n = 8) and 24 h (red curve, n = 6) after AGuIX® NP iv injection during 170 days after tumor implantation. C. Survival curves of 9LGS-bearing rats without treatment (yellow curve, n = 9), only treated by MRT (green curve, n = 15), and treated by MRT 20 min after an injection of 1 M (56 μL) of Dotarem® (blue curve, n = 8), a unique injection of 40 mM (1.4 mL) of Dotarem® (purple curve, n = 8) or AGuIX® NP (red curve, n = 8), 10 days after tumor implantation. D. Intravital two-photon microscopy of labelled AGuIX® NP in subcutaneous B16F10 tumors at 1, 3.5 and 24 h after iv injection, and at the bottom right the corresponding normalized cell fluorescence (CFCT). CFCT was calculated as CFCT = Integrated Density - (Area of selected cell × Mean fluorescence of background reading). E. Kaplan-Meier survival curve comparison obtained for brain B16F10 metastases-bearing mice without treatment (yellow curve, n = 10, including 5 mice injected with AGuIX® NPs without radiation exposure), only treated with 7 Gy radiation exposure (green curve, n = 8), and treated with a combination of AGuIX® NPs (10 mg, 3.5 h after iv injection) and 7 Gy radiation exposure (red curve, n = 9) (p < 0.001). Adapted with permission from ref 36, 39-41, copyrights 2019 Future Medicine Ltd, Nature Publishing Group, Springer Publishing and Ivyspring International Publisher.