Figure 1.

Blood flow recovery is delayed after hindlimb ischemia in SIRT1-Tg mice. (A-F) Representative laser Doppler perfusion images at indicated time point after hindlimb ischemia. (A and B) WT, SIRT1-Tg and SIRT1-Tg mice treated with SIRTl inhibitor EX527 (n= 12). (C and D) WT and SIRT1-KO mice (n= 8 per group). (E and F) WT mice treated with DMSO or SIRTl agonist RSV after hindlimb ischemia (n= 8 per group), Laser Doppler perfusion at various time points was expressed as a ratio of flow between ischemic (L) and sham (R) limbs (L/R). (G) Representative immunofluorescence for CD31 (Scale bars = 100 μm) and α-SMA-positive cells (Scale bars = 20 μm) in the gastrocnemius tissue. (H) Immunostaining of CD31-positive cells represented the relative capillary density. (I) The density of arteriole was expressed as the quantity of arterioles per mm². Data represent mean±SEM. Repeated Measures ANOVA, one-way ANOVA or student's t-test:*P<0.05, **P<0.01 versus the corresponding control.