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. 2020 Jan 1;10(3):1197–1212. doi: 10.7150/thno.39320

Figure 4.

Figure 4

Smooth muscle exosome cZFP609 attenuates hypoxia-induced VEGFA expression in ECs. (A-G and I) qRT-PCR. VSMCs or ECs were exposed to hypoxia for 24 h, except if stated otherwise. (A) RIP assay were performed using HIF1α antibodies in ECs treated with the exosomes of SIRT1-Tg VSMCs under hypoxia. (B and C) cZFP609 expression in VSMCs (B) and the matched exosomes (C). (D) cZFP609 expression in the ECs. (E) cZFP609 expression in the ECs incubated in the VSMC exosome. (F) cZFP609 expression in SIRT1-Tg VSMCs transfected with siScr or sicZFP609. (G) cZFP609 and VEGFA mRNA expression in the ECs treated with the exosome from the cZFP609 siRNA-transfected SIRT1-Tg VSMCs. (H) The sequence of cZFP609 was obtained from Sanger sequencing. (I) cZFP609 and VEGFA mRNA expression. The ECs were transfected with vector and cZFP609 for 24 h. VEGFA protein expression were detected by Western blot (J). (A-G and I) Bar graphs show mean±SEM from 3 independent experiments (n=3). Student's t-test or one-way ANOVA: *P<0.05, **P<0.01 versus the corresponding control.