Figure 7.

cZFP609 negatively regulates blood flow perfusion. (A) Plasma samples were collected from the patients with PAD (n = 19) and control subjects (n = 30). The plasma cZNF609 was determined by qPCR. ABI was calculated by measuring the ankle and the brachial systolic blood pressure using PeriFlux System 5000 (Perimed AB, Datavagen 9A, 175 43 Jarfalla, Sweden). Spearman's correlation analysis was performed. (B) Representative laser Doppler perfusion images at indicated time point after hindlimb ischemia. SIRT1-Tg mice treated with shcZFP609 or shNC (n= 6). (C) Laser Doppler perfusion at various time points was expressed as a ratio of flow between ischemic (L) and sham (R) limbs (L/R). (D) Immunofluorescence for CD31 (Scale bars = 100 μm) and α-SMA-positive cells (Scale bars = 20 μm) in the gastrocnemius tissue. (E) Immunostaining of CD31-positive cells represented the relative capillary density. (F) The density of arteriole was expressed as the quantity of arterioles per mm². Data represent mean±SEM. Repeated Measures ANOVA or student's t-test: *P<0.05, **P<0.01 versus the corresponding control. (G) A working model for SIRT1-Tg VSMCs to impair angiogenesis via inhibition of HIF1α.