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. 2020 Jan 1;10(3):1046–1059. doi: 10.7150/thno.36503

Figure 3.

Figure 3

Knockdown of KLHL2 significantly sensitizes 5'-AZA to inhibit AML cells. MV4-11 cells transfected with KLHL2 shRNA and shcontrol along with UCK1 siRNA or without were treated with or without 5'-AZA for 48 h. (A) CCK8 assays were carried out to examine the proliferation of MV4-11 cells in the presence of different concentrations of 5'-AZA (left panel). The protein levels of UCK1 and KLHL2 were determined (right panel) (B) FACS analysis of the apoptosis of MV4-11 cells under 2.5 uM 5'-AZA. Representative images of cellular apoptosis were shown (left panel). And the percentage of apoptosis cells with indicated treatment was presented in the bar charts (right panel). (C) The percentage of cells with indicated treatment in the different phases of the cell cycle was shown in the bar charts. (D) MV4-11 cells were transfected with a doxycycline-inducible lentiviral vector to upregulating Flag-KLHL2. 24 h later, cells were treated with increasing doses of 5'-AZA. Then cell survival was determined using Annexin V staining (left panel). Cell lysates were analyzed by Western blots using anti-Flag antibody to confirm doxycycline-inducible expression (right panel). *P < .05; **P < .01, and ***P < .001.