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. 2020 Jan 1;10(3):1245–1261. doi: 10.7150/thno.40060

Figure 2.

Figure 2

M1 alleviates PBDE-47-induced mitochondrial impairments and resulting neuronal death. PC12 cells were treated with PBDE-47 (20 μmol/L) in the presence or absence of mitochondrial fusion promoter M1 (5 μmol/L) for 24 h. (A) Representative western blotting and quantification of mitochondrial dynamics proteins. (B) Representative confocal images of mitochondrial morphology and caspase-3-positive puncta. Scale bars, 10 μm (top panel), 2 nm (bottom panel); Red, MitoTracker Deep Red probe staining; blue, DAPI staining. (C) Representative fluorescent images and quantification of MMP. Scale bar, 100 μm. (D) Intracellular ATP levels of PC12 cells. (E) Representative western blotting and quantification of active caspase-3. (F) Cell viability of PC12 cells. Results are expressed as mean ± SD of three separate experiments. *P < 0.05 versus control group; #P < 0.05 versus PBDE-47 group.