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. 2020 Jan 1;10(3):1245–1261. doi: 10.7150/thno.40060

Figure 3.

Figure 3

Mfn2 overexpression alleviates PBDE-47-induced mitochondrial impairments and resulting neuronal death. After infection with adenovirus expressing Mfn2 (Ad-Mfn2) or control adenovirus (Ad-Null) (MOI=300, pre-treated for 24 h), PC12 cells were treated with PBDE-47 (20 μmol/L) for 24 h. (A) Representative western blotting and quantification of mitochondrial dynamics proteins. (B) Representative confocal images of mitochondrial morphology and caspase-3-positive puncta. Scale bars, 10 μm (top panel), 2 nm (bottom panel); Red, MitoTracker Deep Red probe staining; blue, DAPI staining. (C, D) Representative flow cytometry plots and quantification of MMP. (E) Intracellular ATP levels of PC12 cells. (F, G) Representative western blotting and quantification of active caspase-3. (H) Cell viability of PC12 cells. Results are expressed as mean ± SD of three separate experiments. *P < 0.05 versus control group; #P < 0.05 versus PBDE-47 group.