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. 2020 Jan 1;10(3):1245–1261. doi: 10.7150/thno.40060

Figure 6.

Figure 6

Mfn2 and Fis1 double overexpression protects against PBDE-47-induced mitochondrial impairments and resulting neuronal death. After infection with Ad-Fis1 plus Ad-Mfn2 (simultaneous infection) or Ad-Null (all MOI=300, pre-treated for 24 h), PC12 cells were treated with PBDE-47 (20 μmol/L) for 24 h. (A) Representative western blotting and quantification of mitochondrial dynamics proteins. (B) Representative confocal images of mitochondrial morphology and caspase-3-positive puncta in PC12 cell. Scale bars, 10 μm (top panel), 2 nm (bottom panel); Red, MitoTracker Deep Red probe staining; blue, DAPI staining. (C) Representative fluorescent images and quantification of MMP in PC12 cells. Scale bar, 100 μm. (D) Intracellular ATP levels of PC12 cells. (E) Representative western blotting and quantification of active caspase-3. (F) Cell viability of PC12 cells. Results are expressed as mean ± SD of three separate experiments. *P < 0.05 versus control group; #P < 0.05 versus PBDE-47 group.