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. 2020 Jan 1;10(3):998–1015. doi: 10.7150/thno.40076

Figure 6.

Figure 6

ZDHHC17-MAP2K4 Signaling Module is Necessary for GBM Cell Tumorigenic and Invasive Phenotypes. (A, B) Clonogenic survival of (A) U118MG cells transfected with control or ZDHHC17 dsRNA, further transfected with ZDHHC17 plasmid or MAP2K4 dsRNA, or treated with genistein (2.5 μM), and (B) SW1088 cells transfected with control or ZDHHC17 plasmid, further transfected with ZDHHC17 dsRNA or MAP2K4 plasmid, or genistein (2.5 μM) treated and ZDHHC17-expressing. Data represent the means ± SD from three separate experiments (ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001, unpaired t-test). (C-F) Transwell analysis of (C, E) migratory and (D, F) invasive U118MG cells transfected with control or ZDHHC17 dsRNA, or further transfected with ZDHHC17 plasmid or MAP2K4 dsRNA (C, D), and SW1088 cells transfected with control or ZDHHC17 plasmid, or further transfected with ZDHHC17 dsRNA or MAP2K4 plasmid (E, F). Data represent the means ± SD from three separate experiments (ns, not significant; **p < 0.01; ***p < 0.001, unpaired t-test). Scale bar, 500 μm. (G, H) Cell cycle of U118MG cells transfected with control or ZDHHC17 dsRNA, further transfected with ZDHHC17 plasmid or MAP2K4 dsRNA, or genistein (2.5 μM)-treated, as detected by flow cytometry. The percent of cells in the G0-G1 phase, S-phase, and G2-M phase was calculated. Data represent the means ± SD from three separate experiments (*p < 0.05; **p < 0.01; ***p < 0.001, unpaired t-test).