Figure 1.

Sample-to-sample similarity of airway microbiota within baseline periods compared with control samples. Ten baseline periods in six subjects (labeled 1–6) are shown, with multiple baseline periods from the same subject (subjects 2 and 5) labeled a–d. Each point represents the average pairwise Bray-Curtis similarity of that sample to all other samples within that baseline period. Medians and interquartile ranges of all samples within each baseline period and within DNA sequencing control samples (technical replicates of sputum samples [TR1] and generous donor samples [TR2]) are shown. Average pairwise Bray-Curtis similarity of samples is significantly less than average pairwise Bray-Curtis similarity of DNA sequencing control samples within all but one baseline period (P < 0.001 for all baseline periods except baseline 6 [P = 0.262], linear mixed model).