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. Author manuscript; available in PMC: 2020 Jan 13.
Published in final edited form as: Nat Protoc. 2016 Jan 7;11(2):193–213. doi: 10.1038/nprot.2016.009

Figure 1.

Figure 1.

BCR antigen recognition pipeline. This protocol is used to assess whether candidate immunogens can engage and stimulate BCRs of interest, namely germline antibody sequences that give rise to effective humoral responses following infection with pathogen. The protocol is divided into two phases: 1) a rapid screening step wherein antigen interactions with low-affinity germline mIgM are evaluated at the 293F cell surface; and 2) a subsequent analysis of the capacity to stimulate BCR activity through the engagement specificity identified in Phase 1.