Table 1.

BTLA, PD-1 and PD-L1 Expression and Clinical Pathological Factors

Variables	Total, n (%)	BTLA		PD-1		PD-L1
Positive n (%)	P value	Positive n (%)	P value	Positive n (%)	P value
Gender
Male	62(71.3%)	25(40.3%)	0.978	33(53.2%)	0.146	23(37.1%)	0.653
Female	25(28.7%)	10(40.0%)		9(36.0%)		8(32.0%)
Age, mean	61.83
<60	32(36.8%)	14(43.8%)	0.610	13(40.6%)	0.276	14(43.8%)	0.228
≥60	55(63.2%)	21(38.2%)		29(52.7%)		17(30.9%)
Smoking
Non-smoker	29(33.3%)	9 (31.0%)	0.216	10(34.5%)	0.069	5(17.2%)	0.011*
Smoker	58(66.7%)	26(44.8%)		32(55.2%)		26(44.8%)
Sublobe
Left lobe	41(47.1%)	18(43.9%)	0.510	19(46.3%)	0.733	13(31.7%)	0.470
Right lobe	46(52.9%)	17(37.0%)		23(50.0%)		18(39.1%)
Surgery
Standard	83(95.4%)	33(39.8%)	0.683	39(47.0%)	0.273	27(32.5%)	0.006*
Limited	4 (4.8%)	2(50.0%)		3(75.0%)		4(100%)
Pathology
Adenocarcinoma	40(46.0%)	18(45.0%)	0.403	22(55.0%)	0.247	17(42.5%)	0.217
Non-adenocarcinoma	47(54.0%)	17(36.2%)		20(42.6%)		14(29.8%)
Histological grade
G1	20(23.0%)	9(45.0%)	0.754	8 (40.0%)	0.660	6(30.0%)	0.815
G2	44(50.6%)	16(36.4%)		23(52.3%)		16(36.4%)
G3	23(26.4%)	10(43.5%)		11(47.8%)		9(39.1%)
T stage
≤5cm	65(74.7%)	24(36.9%)	0.280	29(44.6%)	0.240	22(33.8%)	0.550
>5cm	22(25.3%)	11(50.0%)		13(59.1%)		9(40.9%)
Lymph node metastasis
No	51(58.6%)	16(31.4%)	0.045*	22(43.1%)	0.254	13(25.5%)	0.019*
Yes	36(41.4%)	19(52.8%)		20(55.6%)		18(50.0%)
Vessel invasion
No	83(95.4%)	32(38.6%)	0.147	39(47.0%)	0.273	29(34.9%)	0.614
Yes	4 (4.6%)	3 (75.0%)		3 (75.0%)		2(50.0%)
Stage
I-II	63(72.4%)	21(33.3%)	0.034*	28(44.4%)	0.247	19(30.2%)	0.084
III	24(27.6%)	14(58.3%)		14(58.3%)		12(50.0%)
TILs percentage
High	23(26.4%)	12(52.2%)	0.393	10(43.5%)	0.524	6(26.1%)	0.501
Medium	30(34.5%)	11(36.7%)		17(56.7%)		11(36.7%)
Low	34(39.1%)	12(35.3%)		15(44.1%)		14 (41.2%)

Note: *Stands for the value of p < 0.05.

Abbreviations: BTLA, B and T lymphocyte attenuator; PD-1, programmed death-1; PD-L1, programmed death ligand-1.