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| Source organism | E. coli |
| DNA source | E. coli strain MG1655 genomic DNA |
| Forward primer | CTATGTCATATGTCTCATACAATCCGTGATAAACAG |
| Reverse primer | GTCATACTCGAGTTATTTGATATATGAATCCAGCAC |
| Cloning vector | pET-22b (ampR) |
| Expression vector | pET-22b |
| Expression host | E. coli DL41 pLysS |
| Complete amino-acid sequence of the construct produced | SHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAAIRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK |
