Table 2. Crystallization.

Method	Hanging-drop vapor diffusion
Plate type	VDX plates (Hampton Research)
Temperature (K)	277
Protein/DNA concentration	15 mg ml−1 RcnR, 10 mM dsDNA (1 RcnR:1.15 dsDNA)
Buffer composition of protein–DNA solution	150 mM NaCl, 10 mM EDTA, 2 mM TCEP, 10% glycerol, 20 mM HEPES pH 7.0
Composition of reservoir solution	21–27% PEG 400, 0.1 M MES pH 6.5
Volume and ratio of drop	1:1 µl
Volume of reservoir (ml)	0.5