Table 2. Crystallization.

	Seed crystals	Diffracting crystals
Method	Vapour diffusion	Vapour diffusion
Plate type	Sitting drop	Sitting drop
Temperature (K)	293	293
Protein concentration (mg ml−1)	14	14
Buffer composition of protein solution	50 mM Tris pH 7.5, 200 mM NaCl, 1 mM EDTA	50 mM Tris pH 7.5, 200 mM NaCl, 1 mM EDTA
Composition of reservoir solution	200 mM calcium acetate, 5–40%(w/v) PEG 3350	200 mM calcium acetate, 20%(w/v) PEG 3350, 20% glycerol
Volume and ratio of drop	200 nl, 1:1	400 nl, 1:1 (+50 nl seeds)
Volume of reservoir (µl)	80	80