Table 1. Macromolecule-production information.

Source organism	S. oralis (ATCC 35037)
DNA source	S. oralis (ATCC 35037)
Forward primer†	5′-TTCGAATTCGATTCTACTACAGAACCTCAGACAAC-3′
Reverse primer‡	5′-TTCGCGGCCGCTCCGGTTTCAGGTACACTGTTCTTATTATTCG-3′
Cloning vector	pET-32a(+) (Novagen)
Expression vector	pET-32a(+) (Novagen)
Expression host	E. coli BL21(DE3) pLysS
Complete amino-acid sequence of the construct produced§	MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA GSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEFDSTTEPQTTLHKTITPISGQDDKYELSLDITSKLGTETQTDPLDVVLVADLSGSMQNQDVQSFDGRTISRIDALKNTLRGTNGRKGLIDTILSNSNNRLSMVGFGGKIDNKKVDQYWDGNKWRLFRPYWPYERMTKYYDGVSPWDDANTILGWSNNARAAKTAVYNMSIAGGNSIGTESGIGTGTNIGAGLTLANQLMGSARSNAKKVVILLSDGFANMVYDANGYTIYNYNNEDPNIETAPQWFWDRLNNNLNSLSYSLAPTLDGFYSIKFRYSNNVDSITSLQYYMRQHNASIPNEIFSANDEDQLRDSFKDITDKILPLGIHHVSIKDVLSKYVQLLPNGSSEFRVVKEKDGSSEILTENQVTFDTKTTSEGLVEVTAKFSPNYSLEDGARYVLKFTVTSSQEALDAIAGDKKLEAGDAEGSDVNKLYSNKGASVTYSYGIGNSQTKTKEYSDNPTFKPSDPLTVPVEVEWQGVTGARTVITADQPSNVELKLVQKNKNGGSDNQDYRKTNVNVSKNVSNETRNFEKVAKGYQYDLIAPDVPAFTKEIKNVGTESNPSFKVIYKQLPSLTIKKVLEAENNLNKEFRIKVKLTSPDSKPLNGTFGEITVVNGEAEIRVEKRKRWRGILSYLPRGTHYKVEEEAASTNGYHVTYENQEGDLNKDETSTVTNHKLPSLSVTKKVTGVFANLLKSFKITINIRDAQNSPLNGTYTATVNNKRTPLQFTNGRASIDLNKDQTIKIDGLPLDSHYTVEEETNSSRGYQVSYENQEGKLDGDKSATVTNNKNSVPETCGR

^†The EcoRI site is underlined.

^‡The NotI site is underlined.

^§Cloning artefacts are underlined or coloured. (Trx tag, red; hexahistidine tag, blue; thrombin recognition site, pink; S-tag, green; enterokinase recognition site, brown.)