| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
VDX plate (Hampton Research) |
VDX plate (Hampton Research) |
| Temperature (K) |
295 |
295 |
| Protein concentration (mg ml−1) |
30 |
40 |
| Buffer composition of protein solution |
20 mM HEPES pH 8.0, 150 mM NaCl |
20 mM HEPES pH 8.0, 150 mM NaCl |
| Composition of reservoir solution |
1 M potassium iodide, 25% PEG 3350, 100 mM HEPES pH 7.8 |
0.2 M ammonium acetate pH 7.2, 20% PEG 3350 |
| Volume and ratio of drop |
2 µl, 1:1 |
2 µl, 1:1 |
| Volume of reservoir (ml) |
1 |
1 |
