| Method |
Sitting-drop vapor diffusion |
Hanging-drop vapor diffusion |
| Plate type |
96-well 2-drop MRC Crystallization Plates (Molecular Dimensions) |
24-well VDX plate (Hampton Research) |
| Temperature (K) |
293 |
293 |
| Protein concentration (mg ml−1) |
15 |
10 |
| Buffer composition of protein solution |
50 mM MES, 50 mM NaCl, 5 µM ZnSO4, 10 mM DTT pH 6.5 |
50 mM MES, 50 mM NaCl, 5 µM ZnSO4, 10 mM DTT pH 6.5 |
| Composition of reservoir solution |
0.4 M lithium sulfate monohydrate, 0.1 M bis-Tris pH 6.5, 25%(w/v) PEG 3350 |
4%(v/v) Tacsimate pH 6.0, 12%(w/v) PEG 3350 |
| Volume and ratio of drop |
2 µl (1:1 ratio) |
2 µl (1:1 ratio) |
| Volume of reservoir (µl) |
100 |
1000 |
