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. 2020 Jan 13;15(1):e0227568. doi: 10.1371/journal.pone.0227568

Fig 4. The regulatory effects of ROR1-AS1 on miR-504.

Fig 4

(A) RT-qPCR analysis of miR-504 expression in 65 cases of bladder cancer samples. (B) The correlation of miR-504 and ROR1-AS1 expression in bladder cancer samples was evaluated by Pearson’s correlation analysis. (C) Sequences of ROR1-AS1 binding sites within the miR-504. (D) The miR-504 expression in T24 and 5637 cells was detected by RT-qPCR after transfection with miR-504 inhibitor or inhibitor NC. (E) Luciferase assay was measured 24 h post transfection in bladder cancer cells, which were co-transfected with ROR1-AS1-WT and ROR1-AS1-MUT vectors, together with miR-504 inhibitor or inhibitor NC. (F) Transfection of shRNA-ROR1-AS1 into bladder cancer cells showed upregulation of miR-504 expression, but co-transfection with shRNA-ROR1-AS1 and miR-504 inhibitor reversed these effects. Data were presented as the mean ± SD (n = 3). *p < 0.05.