Table 2. Summary of end point measurements and T. pallidum yield where applicable for in vivo rabbit propagation of patient specimens.
| Specimen ID | Inoculationroute1 | Passage number2 | Measurement | T. pallidum yield3 (qPCR) | DF | T. pallidum strain type4 | |||
|---|---|---|---|---|---|---|---|---|---|
| Orchitis | Serology | PCR (blood) | PCR (testes) | ||||||
| CDC-SF011 | IT | 1 | - | - | - | + | 64.29/ml | - | ND6 |
| IT | 2 | - | +5 | - | - | - | - | ||
| CDC-SF002 | IT | 1 | - | - | - | - | - | - | N/A |
| CDC-SF003 | IV and IT | 1 | + | + | + | + | 4.84 x 106/ml | + | 14d9f |
| IT | 2 | + | + | + | + | 1.62 x 106/ml | + | ||
| CDC-SF004 | IV and IT | 1 | - | - | - | - | - | - | N/A |
| CDC-SF005 | IV and IT | 1 | - | - | - | - | - | - | N/A |
| CDC-SF006 | IV and IT | 1 | - | - | - | - | - | - | N/A |
| CDC-SF007 | IV and IT | 1 | - | + | + | + | 6.91 x 105/ml | + | 14d9g |
| IT | 2 | - | + | + | + | 3.11 x 104/ml | + | ||
| CDC-SF008 | IV and IT | 1 | - | + | - | + | 1.06 x 104/ml | - | 14d10c7 |
| IT | 2 | - | - | - | + | 1.1 x 103/ml | - | ||
1 IT–intratesticular, left testis; IV–intravenous.
2 Indicates passage number for serial passages performed.
3 T. pallidum yield expressed as genomic equivalents in left testis extract.
4 Strain typing was performed on testes samples that yielded PCR+ results.
5 Weakly positive TP-PA titer +/- 1:80
6 Isolate could not be typed due to low number of spirochetes after rabbit propagation
7 Strain type observed with residual lesion swab specimen in NRS