Fig 7.

Effect of miR-71 suppression in miR-71 level (A), and in the mRNA level of the potential target genes EmuJ_000085700 (frizzled) (B), EmuJ_000440000 (T cell immunomodulatory protein) (C) and EmuJ_000250800 (serine:threonine protein kinase) (D). Primary cells were obtained from Echinococcus multilocularis metacestodes (Ingrid isolate). The cells were electroporated with anti-miR-71-2´OMe or scrambled-2´OMe and their effects on the levels of endogenous miRNA (A) and predicted target mRNAs (B,C,D) determined by RT-qPCR at 24 h post-electroporation (48 h cell culture). Data illustrate representative results with the mean and standard error derived from triplicate experiments. * mean P ≤ 0.05.