Table 1. Sixteen potential target genes of miR-71 in Echinocuccus multilocularis primary cell culture up-regulated after transfection with anti-miR-71-2´OMe.
(log2FoldChange_Shrinkage > 0.48 and P-value adjustment < 0.05). Position of miR-71 target site in the 3’ UTR of each target gene, conservation of miR-71 targeting in Echinococcus canadensis, Echinococcus granulosus and Taenia solium orthologs and type of site according to Bartel [14] are also shown.
| Gene ID | Annotation | FoldChange | UTR_start | UTR_end | Conservation | Type of site |
|---|---|---|---|---|---|---|
| EmuJ_000085500 | Expressed conserved protein | 1.4 | 14 | 33 | T. solium, E. canadensis and E. granulosus | 7mer-A1 |
| EmuJ_000085700 | Frizzled | 1.7 | 156 | 180 | T. solium and E. granulosus | 7mer-m8 |
| EmuJ_000124300 | CTR1, high affinity copper uptake protein 1 | 1.5 | 27 | 50 | T. solium, E. canadensis and E. granulosus | 8mer |
| EmuJ_000145900 | Glutamate receptor interacting protein 1 | 1.6 | 5 | 26 | T. solium | 8mer |
| EmuJ_000154000 | E3 ubiquitin protein ligase MARCH8 | 1.5 | 33 | 54 | T. solium, E. canadensis and E. granulosus | 7mer-m8 |
| EmuJ_000165500 | USP6 N terminal protein | 1.4 | 23 | 45 | T. solium, E. canadensis and E. granulosus | 8mer |
| EmuJ_000191100 | Transcription factor SOX 14 | 1.6 | 54 | 74 | T. solium, E. canadensis and E. granulosus | 8mer |
| EmuJ_000228700 | Leucine rich repeat, typical subtype | 1.6 | 186 | 206 | T. solium, E. canadensis and E. granulosus | 7mer-m8 |
| EmuJ_000250800 | Serine:threonine protein kinase | 1.4 | 218 | 239 | E. granulosus | 7mer-m8 |
| EmuJ_000440000 | T cell immunomodulatory protein | 1.5 | 26 | 47 | T. solium, E. canadensis and E. granulosus | 8mer |
| EmuJ_000440000 | T cell immunomodulatory protein | 1.5 | 84 | 106 | E. canadensis and E. granulosus | 7mer-m8 |
| EmuJ_000473600 | Sodium dependent phosphate transport protein 1 | 1.4 | 154 | 174 | T. solium and E. granulosus | 7mer-m8 |
| EmuJ_000610700 | PAB dependent poly(A) specific ribonuclease | 1.5 | 25 | 49 | n/c 2 | 8mer |
| EmuJ_000848200 | Protocadherin alpha 7 | 1.7 | 9 | 32 | n/c | 7mer-m8 |
| EmuJ_000865800 | n/a1 | 2.1 | 12 | 33 | n/c | 8mer |
| EmuJ_001140200 | Expressed conserved protein | 2.8 | 169 | 191 | n/c | 8mer |
| EmuJ_001186600 | Ets variant gene 4 | 1.6 | 25 | 46 | T. solium | 7mer-m8 |
1 Without annotation in Wormbase parasite
2 Without conservation in T. solium, E. canadensis and E. granulosus