Fig 3. Validation of the Rule-based Model.

Bold traces (A-C) and solid circles (D) are the average of N = 50 executions. For each species (A-C), six representative traces are also shown (semi-transparent lines). (A) Model output resulting from stimulation with a large continuous bolus of Ca²⁺/CaM. Concentrations of active (red), initially CaM-bound (yellow), fully CaM-bound (blue), and pThr-286 (cyan) subunits. (B) Time-course average concentration (bold trace) of active subunits stimulated by 5 Hz or 50 Hz Ca²⁺/CaM. (C) Time-course concentration of pThr-286 subunits stimulated continuously by 5 Hz or 50 Hz Ca²⁺/CaM. (D) Frequency-dependent activation (red) and pThr-286 (cyan) of CaMKII subunits, with SEM error bars. Black dotted traces are linear fits.