Fig 3. Emergency granulopoiesis and neutrophil supply is not impaired in Il23a^-/- mice.

WT and Il23a^-/- mice were infected intravenously with 2x10⁵ CFU C. albicans and treated with BrdU for the last 12h prior to analysis of neutrophil proliferation by flow cytometry at 48h post infection. (A) Schematic outline of the experimental setup. (B) Representative FACS plots show the gating strategy for identifying BrdU⁺ neutrophils in the BM. Neutrophils were defined as shown in Fig 2C. (C, D) Summary graphs show percentage of BrdU⁺ cells in the total population of BM (C) or kidney neutrophils (D). (E) G-CSF levels in the serum were quantified by ELISA at 48h post infection. In C-E, bars are the mean + SD of each group with n = 3. Data are representative of two independent experiments. Statistics were calculated using unpaired Student’s t-Test. *p<0.05, **p<0.01, ***p<0.001.