Figure 8.

Unsupervised clustering of AD patients and healthy controls across all principal components of the blood flow cytometry marker frequencies (%) by k-means. In AD, the frequencies of different markers defined three meaningful age-clusters, aligning along a spectrum. While infants (pink ellipse) clustered on the far left, and adults (green ellipse) on the right, children and adolescents (blue ellipse) clustered together between the other age cohorts. The markers that best distinguished between clusters appear in the boxes between two cohorts (colors of markers parallel the colors of the relative age group). Arrows designate elevated frequencies of a given marker among the specific age group. In healthy controls, clusters did not clearly align patients along an age spectrum. CLA, Cutaneous lymphocyte antigen; ICOS, Inducible co-stimulator molecule; Tregs, T-regulatory cells.