KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-CD3 (145-2C11) | Thermo Fisher Scientific | Cat# 16-0031-82; RRID: AB_468847 |
| anti-CD28 (37.51) | Thermo Fisher Scientific | Cat# 16-0281-82; RRID: AB_468921 |
| anti-Armenian and Syrian Hamster IgG | BD Biosciences | Cat# 554009; RRID: AB_395205 |
| anti-CD4 (RM4.5) | BioLegend | Cat# 100536; RRID: AB_493701 |
| anti-CD8 (53-6.7) | BioLegend | Cat# 100730; RRID: AB_493703 |
| anti-CD44 (IM7) | BioLegend | Cat# 103020; RRID: AB_493683 |
| anti-CD62L (MEL-14) | BioLegend | Cat# 104418; RRID: AB_313103 |
| anti-CD45.1 (A20) | BioLegend | Cat# 110728; RRID: AB_893346 |
| anti-CD45.2 (104) | BioLegend | Cat# 109808; RRID: AB_313445 |
| anti-Vα2 (B20.1) | BioLegend | Cat# 127810; RRID: AB_1089250 |
| anti-TCRβ (H57-597) | BioLegend | Cat# 109220; RRID: AB_893624 |
| anti-B220 (RA3-6B2) | BioLegend | Cat# 103212; RRID: AB_312997 |
| anti-KLRG1 (2F1) | SouthernBiotech | Cat# 1807-09; RRID: AB_2795369 |
| anti-IL7Rα (A7R34) | BioLegend | Cat# 135022; RRID: AB_1937273 |
| anti-CXCR5 (2G8) | BD Biosciences | Cat# 551960; RRID: AB_394301 |
| Cat# 561988; RRID: AB_10893355 | ||
| anti-CD27 (LG.3A10) | BioLegend | Cat# 124233; RRID: AB_2687192 |
| anti-CD69 (H1.2F3) | BioLegend | Cat# 104522; RRID: AB_2260065 |
| anti-CXCR6 (SA051D1) | BioLegend | Cat# 151109; RRID: AB_2616760 |
| anti-SLAM (TC15-12F12.2) | BioLegend | Cat# 115922; RRID: AB_2303663 |
| anti-CD107a (1D4B) | BioLegend | Cat# 121626; RRID: AB_2572055 |
| anti-IFNγ (XMG1.2) | BioLegend | Cat# 505810; RRID: AB_315404 |
| anti-TNFα (MP6-XT22) | BioLegend | Cat# 506324; RRID: AB_2256076 |
| anti-IL-2 (JES6-5H4) | BioLegend | Cat# 503808; RRID: AB_315302 |
| anti-Granzyme B (GB11) | BD Biosciences | Cat# 562462; RRID: AB_2737618 |
| anti-p-ERK (T202/Y204; 20A) | BD Biosciences | Cat# 562644; RRID: AB_2737698 |
| anti-p-AKT (S473; M89-61) | BD Biosciences | Cat# 561670; RRID: AB_10896328 |
| anti-CD3 (HIT3a) | BD Biosciences | Cat# 740073; RRID: AB_2687538 |
| anti-CD4 (RPA-T4) | BD Biosciences | Cat# 555347; RRID: AB_395752 |
| anti-CD8 (RPA-T8) | BD Biosciences | Cat# 555366; RRID: AB_395769 |
| anti-CCR7 (G043H7) | BioLegend | Cat# 353212; RRID: AB_10916390 |
| anti-CD45RA (HI100) | BioLegend | Cat# 304134; RRID: AB_2563814 |
| anti-SIRT1 (D60E1) | Cell Signaling Technology | Cat# 3931; RRID: AB_1642293 |
| anti-DUSP6 (EPR129Y) | Abcam | Cat# ab76310; RRID: AB_1523517 |
| anti-b-actin (13E5) | Cell Signaling Technology | Cat# 4970; RRID: AB_2223172 |
| anti-Rabbit IgG HRP | Cell Signaling Technology | Cat# 7074; RRID: AB_2099233 |
| Bacterial and Virus Strains | ||
| LCMV Armstrong | Laboratory of Rafi Ahmed | Ahmed et al., 1984 |
| Listeria-GP61-80 (Lm-gp61) | Laboratory of Rafi Ahmed | Williams et al., 2008 |
| Biological Samples | ||
| Peripheral blood from Individuals previously recovered from WNV infection | University of Arizona College of Medicine | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fixable Viability Dye | Thermo Fisher Scientific | Cat# 65-0866-14 |
| PE Streptavidin | BioLegend | Cat# 405204 |
| IAb LCMV GP 66–77 (DIYKGVYQKSV) tetramer | NIH tetramer core facility | N/A |
| Db LCMV GP 33–41 (KAVYNFATM) tetramer | NIH tetramer core facility | N/A |
| Db LCMV GP 276–286 (SGVENPGGYCL) tetramer | NIH tetramer core facility | N/A |
| Db LCMV NP 396–404 (FQPQNGQFI) tetramer | NIH tetramer core facility | N/A |
| HLA-A*02 WNV Env 430–438 (SVGGVFTSV) tetramer | NIH tetramer core facility | N/A |
| DRB1*01:01 WNV NS1 205–220 (RLNDTWKLERAVLGEVK) tetramer | NIH tetramer core facility | N/A |
| DRB1*04:01 WNV NS1 205–220 (RLNDTWKLERAVLGEVK) tetramer | NIH tetramer core facility | N/A |
| LCMV GP61–80 peptide (GLKGPDIYKGVYQFKSVEFD) | Anaspec | Cat# AS-64851 |
| LCMV GP33–41 peptide (KAVYNFATM) | Anaspec | Cat# AS-61296 |
| LCMV GP276–286 peptide (SGVENPGGYCL) | Anaspec | Cat# AS-62539 |
| LCMV NP396–404 peptide (FQPQNGQFI) | Anaspec | Cat# AS-61700 |
| BD GolgiStop | BD Biosciences | Cat# 554724 |
| Critical Commercial Assays | ||
| Naive CD4+ T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat# 130-104-453 |
| CD8a+ T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat# 130-104-075 |
| Pan T Cell Isolation Kit II, mouse | Miltenyi Biotec | Cat# 130-095-130 |
| Anti-Biotin MicroBeads | Miltenyi Biotec | Cat# 130-090-485 |
| Fixation/Permeabilization Solution Kit | BD Biosciences | Cat# 554714 |
| BD Cytofix Fixation Buffer | BD Biosciences | Cat# 554655 |
| BD Phosflow Perm Buffer III | BD Biosciences | Cat# 558050 |
| RNeasy Plus Micro Kit | QIAGEN | Cat# 74034 |
| miRNeasy Micro Kit | QIAGEN | Cat# 217084 |
| miRCURY LNA RT Kit | QIAGEN | Cat# 339340 |
| TaqMan Universal Master Mix II | Thermo Fisher Scientific | Cat# 4440047 |
| TaqMan MicroRNA Reverse Transcription Kit | Thermo Fisher Scientific | Cat# 4366596 |
| TaqMan MicroRNA Assays, miR-181a | Thermo Fisher Scientific | Assay ID 000480 |
| TaqMan MicroRNA Assays, snoRNA202 | Thermo Fisher Scientific | Assay ID 001232 |
| SMARTer® RACE 5’/3’ Kit | Takara Bio USA, Inc. | Cat# 634859 |
| MiSeq Reagent Kit v3 | Illumina | Cat# MS-102-3003 |
| Deposited Data | ||
| TRB sequencing data | This paper | SRA: PRJNA558782 |
| Experimental Models: Cell Lines | ||
| BHK cells | ATCC | ATCC CCL-10 |
| Vero cells | ATCC | ATCC CCL-81 |
| Plat-E cells | Cell Biolabs, Inc. | Cat# RV-101 |
| Experimental Models: Organisms/Strains | ||
| C57BL6/J mice | Jackson Laboratory | Stock No: 000664 |
| distal Lck-Cre mice | Jackson Laboratory | Stock No: 012837 |
| Rosa26-YFP mice | Jackson Laboratory | Stock No: 006148 |
| miR-181ab1fl/fl mice | Laboratory of CZ Chen | Fragoso et al., 2012 |
| SMARTA TCR transgenic mice | Laboratory of Rafi Ahmed | Williams et al., 2008 |
| Oligonucleotides | ||
| hsa-miR-181a-5p miRCURY LNA miRNA PCR Assay | QIAGEN | Cat# YP00206081 |
| U6 snRNA(hsa, mmu) miRCURY LNA miRNA PCR Assay | QIAGEN | Cat# YP00203907 |
| SNORD48(hsa) miRCURY LNA miRNA PCR Assay | QIAGEN | Cat# YP00203903 |
| TRB amplicon; forward primer 5’-GGCATTCCTGC TGAACC GCTCTTCCGATCTNNNNACTAGGTAAAGCAGTGGTATCAA CGCAGAGT-3’ | This paper | N/A |
| TRB amplicon; reverse primer 5’-ACACTCTTTCCCTACAC GACGCTCTTCCGATCTNNNNxxxxxxxxTGGCTCAAACAA GGAGACCT-3’ | This paper | N/A |
| Dusp6 shRNA sequence; 5’-TGCTGTTGACAGTGAGCGAACA CATCGAATCTGCCATTAATAGTGAAGCCACAGATGTATTAA TGGCAGATTCGATGTGTGTGCCTACTGCCTCGGA-3’ | This paper | N/A |
| miR30 common; forward primer 5’-CAGAAGGCTCGAGAAGG TATATTGCTGTTGACAGTGAGCG-3’ | This paper | N/A |
| miR30 common; reverse primer 5’-CTAAAGTAGCCCCTTGA ATTCCGAGGCAGTAGGCA-3’ | This paper | N/A |
| Recombinant DNA | ||
| LMPd-shCtrl vector | Laboratory of YS Choi | Choi and Crotty, 2015 |
| LMPd-shDusp6 vector | This paper | N/A |
| Software and Algorithms | ||
| FlowJo | TreeStar | RRID:SCR_008520 |
| Prism | GraphPad Software | RRID:SCR_002798 |
| IMGT | Lefranc and Lefranc, 2001 | http://www.imgt.org/ |
| MiXCR | Bolotin et al., 2015 | https://mixcr.readthedocs.io/en/master/ |