Figure 4.

Effect of the nPKCδ inhibitor rottlerin on the expression of adhesion molecules in HaCaT keratinocytes. (a) Cells were treated with various concentrations of rottlerin for 48 h, then harvested and similar amounts of proteins were subjected to Western immunoblotting to detect the adhesion molecules Dsg1, Dsg3 and P-cad. To assess equal loading, nitrocellulose membranes were stripped and re-probed with an anti-β-actin antibody (β-act). The figure is a representative of four to five experiments yielding similar results. (b) The amounts of the adhesion molecules were quantitated by densitometry (optical density; OD), then normalized to those of β-actin, and finally expressed as the percentage of the vehicle-treated (control, C) cells regarded as 100%. Points represent the mean ± SEM of four to five independent experiments. Asterisks mark significant (P < 0.05) differences, defined by Student’s t-test, compared with control.