Fig. 2.

Rspo2 and Wnt1 over-expression have contrasting effects on cell growth in monolayer culture. Growth curves of parental cell lines and stable transfectants expressing either Rspo2 or Wnt1 alone or in combination were generated as described in the Materials and Methods Section (upper part C57MG, lower part HC11). Cells were seeded in 24-well plates (1,000 cells/well), and were trypsinized and counted at 24 or 48 h intervals. Each counting was done in triplicate and expressed as the mean ± SD.