Skip to main content
. Author manuscript; available in PMC: 2020 Jan 13.
Published in final edited form as: J Cell Physiol. 2012 May;227(5):1960–1971. doi: 10.1002/jcp.22924

Fig. 5.

Fig. 5.

Rspo2 derived tumors show EMT signature. A: Relative expression of the mRNAs encoding EMT-related genes: E-cadherin, Cytokeratin 18 (Krt18), Laminin-1, ZO-1, Snail, Slug, Twist, Vimentin, N-cadherin, Fibronectin1, TGFb1, and SMA in Rspo2, Wnt1, Rspo2/Wnt1, and Notch1 tumors as well as in cultured parental HC11 cells, determined by real-time PCR. GAPDH mRNA was used to normalize the variability in template loading. The data are reported as the means ± SD from one representative tumor from each group analyzed in three repeats. B: Immunohistochemical staining of Rspo2, Wnt1, and Rspo2/Wnt1 tumors for Cytokeratin 18 (Krt18), E-cadherin, Vimentin, Cytokeratin 14 (Krt14), SMA, and Slug. The original magnification was 400×.