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. Author manuscript; available in PMC: 2020 Jan 13.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Feb 13;485(2):446–453. doi: 10.1016/j.bbrc.2017.02.058

Fig. 1.

Fig. 1.

Expression and selection of the yeast display CH2 domain library. (A) The grafted CH2 yeast library indicated 43% of expressed cell. In contrast, the grafted VH yeast library indicated ~20% of expressed cell. (B) The sorting rounds of CH2 yeast library against nucleolin. The double-positive yeast cells were selected from sort gates. Four figures shows sorting of yeast library against nucleolin at rounds 1,2,3, and 4. (C) Binding of yeast-expressed CH2 binders to antigens. Three antigens nucleolin, folate receptor b, and mesothelin, were used to detect CH2 binders.