Figure 1.
Binding and competition ELISA. A, binding of IgG1 m610.27 and m630.3Fc to hIGF-II, hIGF-I, and human insulin. B, binding of the antibodies to mIGF-II. C, competition of m630.3Fc with IgG1s m610.27 and m708.5 in binding to hIGF-II. D, competition of m630.3Fc with IgG1s m610.27 and m708.5 in binding to mIGF-II. E, binding of m660 to hIGF-II. F, binding of m660 to long hIGF-II. G, competition of the antibodies with IGFBP2 in binding to hIGF-II. H, competition of the antibodies with IGFBP3 in binding to hIGF-II. All assays were done in duplicate. Error bars were included in all binding curves.