Table 2. Cryo-EM structure determination statistics.

	E46K mutant	Phosphorylated	N-terminal acetylated	α-Syn polymorph 2a	α-Syn polymorph 2b
Data Collection
Pixel size [Å]	0.831	0.831	0.831	0.629	0.629
Defocus Range [µm]	−0.8 to −2.5	−0.8 to −2.5	−0.8 to −2.5	−0.8 to −2.5	−0.8 to −2.5
Voltage [kV]	300	300	300	300	300
Exposure time [s per frame]	0.2	0.2	0.2	0.2	0.2
Number of frames	50	50	50	50	50
Total dose [e-/Å2]	69	69	69	69	69
Reconstruction
Box width [pixels]	280	280	280	280	280
Inter-box distance [pixels]	28	28	28	28	28
Micrographs	843	1’887	948	1’143	1’143
Manually picked fibrils	2’702	5’095	3’751	5’233	5’233
Initially extracted segments	65’893	107’144	43’276	100’323	100’323
Segments after 2D classification	50’514	107’126	35342	100’193	100’193
Segments after 3D classification	50’514	21’685	35342	19’937	3’989
Resolution after 3D refinement [Å]	4.65	4.65	4.75	3.34	3.75
Final resolution [Å]	4.56	4.31	4.39	2.99	3.39
Estimated map sharpening B-factor [Å2]	−208.6	−104.3	−178.1	−67.1	−76.4
Helical rise [Å]	4.85	4.84	4.78	4.80	2.40
Helical twist [°]	−0.79	−0.77	−0.71	−0.80	179.55