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. 2019 Dec 18;127(12):127006. doi: 10.1289/EHP5139

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EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

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Figure 1A is a bar graph with standard deviation plotting cell proliferation (of control; y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). Figure 1B is a bar graph with standard deviation plotting LDH release (of control; y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). Figure 1C is a bar graph with standard deviation plotting fluorescence intensity (of control; y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). Figure 1D displays representative images for DAPI, ROS, and Merge. — Cell proliferation, cytotoxicity and ROS production after treatment with MeHg for $0.5 h$ in astrocytes. (A) Cell proliferation in astrocytes was measured using the MTT assay. (B) Cytotoxicity in astrocytes was detected via LDH release. (C) Effects of MeHg treatment on ROS production using the fluorescent probe DCFH-DA. The fluorescence intensity was detected by the microplate reader. (D) Representative images for ROS generation induced by MeHg ( $10 μ M$ , $0.5 h$ ) treatment. $Scale bar = 100 μ m$ . $H_{2} O_{2}$ ( $6 μ M$ , $0.5 h$ ) was set as a positive control for ROS generation. Green fluorescence indicates ROS and blue (DAPI) indicates the nucleus. Note: Data are presented as $mean \pm SD$ from three independent experiments ( $n = 3$ ). con, control (culture medium treatment without MeHg); DAPI, 4′,6-diamidino-2-phenylindole; DCFH-DA, 2′,7′-dichlorofluorescin diacetate; $H_{2} O_{2}$ , hydrogen peroxide; LDH, lactate dehydrogenase; MeHg, methylmercury; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl diphenyltetrazolium bromide; ROS, reactive oxygen species. * $p < 0.05$ vs. control by one-way analysis of variance (ANOVA) with Dunnett’s post hoc test.