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. 2019 Dec 18;127(12):127006. doi: 10.1289/EHP5139

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EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

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Figure 2A comprises a Western blotting for HIF 1 alpha (120 kilo Dalton) and beta actin (43 kilo Dalton) and a bar graph with standard deviation plotting HIF 1 alpha or beta actin (y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). Figure 2B comprises a Western blotting for HIF 1 beta (87 kilo Dalton) and beta actin (43 kilo Dalton) and a bar graph with standard deviation plotting HIF 1 beta or beta actin (y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). Figure 2C comprises a Western blotting for GLUT 1 (55 kilo Dalton), EPO (21 kilo Dalton), VEGF A (17 kilo Dalton), and beta actin (43 kilo Dalton), as well as a bar graph with standard deviation plotting relative protein expression per beta actin (y-axis) across treatment with 0, 1, 2.5, 5, and 10 micromolar of methylmercury (x-axis). — Effects of MeHg on the expression of $HIF- 1 α - related$ proteins in astrocytes. (A) Western blotting for $HIF- 1 α$ in astrocytes following MeHg (0, 1, 2.5, 5 or $10 μ M$ , $0.5 h$ ) exposure. (B) Western blotting for $HIF- 1 β$ in astrocytes following MeHg (0, 1, 2.5, 5, or $10 μ M$ , $0.5 h$ ) exposure. (C) Effect of MeHg (0, 1, 2.5, 5, or $10 μ M$ , $0.5 h$ ) exposure on the expression of the downstream proteins of $HIF- 1 α$ , including GLUT-1, EPO, and VEGF-A. $β -actin$ was used as the internal control. Note: Data are presented as $mean \pm SD$ from three independent experiments ( $n = 3$ ). con, control (culture medium treatment without MeHg); EPO, erythropoietin; GLUT-1, glucose transporter 1; $HIF- 1 α$ , Hypoxia-inducible $factor- 1 α$ ; $HIF- 1 β$ , Hypoxia-inducible $factor- 1 β$ ; MeHg, methylmercury; VEGF-A, vascular endothelial growth factor A. * $p < 0.05$ vs. control by one-way ANOVA with Dunnett’s post hoc test.