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. 2019 Dec 18;127(12):127006. doi: 10.1289/EHP5139

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Figure 5A comprises a Western blotting for HIF 1 alpha (120 kilo Dalton) and beta actin (43 kilo Dalton) and a bar graph with standard deviation plotting HIF 1 alpha or beta actin (of control; y-axis) across treatment with NAC and micromolar of methylmercury (x-axis). Figure 5B is a bar graph plotting cell proliferation (of control; y-axis) across treatment with NAC and micromolar of methylmercury (x-axis). Figure 5C comprises a Western blotting for HIF 1 alpha (120 kilo Dalton) and beta actin (43 kilo Dalton) and a bar graph with standard deviation plotting HIF 1 alpha or beta actin (of control; y-axis) across treatment with Trolox and micromolar of methylmercury (x-axis). Figure 5D is a bar graph plotting cell proliferation (of control; y-axis) across treatment with Trolox and micromolar of methylmercury (x-axis). — $HIF- 1 α$ protein expression and cell proliferation after pre-treatment with antioxidants in MeHg-treated astrocytes. (A) Effect of NAC pretreatment ( $2.5 mM$ , $4 h$ ) on $HIF- 1 α$ protein expression. (B) Effect of NAC pretreatment on the decreased cell proliferation induced by MeHg. (C) Effect of Trolox pretreatment ( $2.5 mM$ , $4 h$ ) on $HIF- 1 α$ protein expression. (D) Effect of Trolox pretreatment on the MeHg-induced decrease in cell proliferation. Note: Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. Data are presented as the $mean \pm SD$ from three independent experiments ( $n = 3$ ). con, control (culture medium treatment without MeHg); $HIF- 1 α$ , Hypoxia-inducible $factor- 1 α$ ; MeHg, methylmercury; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl diphenyltetrazolium bromide; NAC, N-acetyl-cysteine, Trolox, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. * $p < 0.05$ vs. control, ^# $p < 0.05$ vs. MeHg-only group.