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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Pharm. 2019 May 30;16(7):3024–3039. doi: 10.1021/acs.molpharmaceut.9b00263

Figure 4.

Figure 4.

Rapa reduces lymphocytic infiltration in the LG of male NOD mice. (A) One of each pair of LGs from each mouse in the cohort was collected at the conclusion of the study. The 25th, 50th, and 75th percentile sections from each LG were quantified by three blinded observers to determine the average percentage area of infiltrate per gland (n = 15). (B) Inflamed LGs show areas of purple nuclear staining, which indicate foci of infiltrating lymphocytes (outlined in blue). Lymphocytic infiltration was reduced by FAF–Rapa (middle panel). The scale bar represents 200 μm. (C) The percentage area of infiltration was calculated using ImageJ (Mean ± SD).