Table 1.
Biophysical Characteristics of FAF, FAF–Rapa, and Rhodamine-Labeled FAF–Rapa
| Rh (nm)c at | ||||
|---|---|---|---|---|
| label | formulation compositiona | MW (kDa)b | 20 °C | 37 °C |
| FAF | FKBP–G(VPGAG)192–FKBP | 96.6 | 8 ± 1 | 8 ± 1 |
| FAF–Rapa | Rapa/FKBP–G(VPGAG)192–FKBP/Rapa | 98.3 | 9 ± 2 | 8 ± 0.3 |
| Rho–FAF–Rapa | rhodamine–[Rapa/FKBP–G(VPGAG)192–FKBP/Rapa] | 98.8 | 9 ± 1 | 7 ± 0.3 |
a
FKBP amino acid sequence: GVQVETISPGDGRTFPKRGQT CVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE. G(VPGAG)192Y is the amino acid sequence of the elastin-like polypeptide, A192.
b
Expected molecular weights for FAF–Rapa and Rho–FAF–Rapa were reported based on observed 1:1 molar ratios of Rapa to FKBP and the rhodamine-labeling ratio.
c
Hydrodynamic radii of each formulation (of 10 μM, PBS) were determined in triplicate by DLS (Mean ± SD).