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. 2019 Sep 11;317(6):L758–L767. doi: 10.1152/ajplung.00224.2019

Fig. 3.

Fig. 3.

Bcl-2 adenovirus E1B 19 kDa-interacting protein 3 (Bnip3) downregulation negatively affects human airway smooth muscle (ASM) cell adhesion and spreading on tissue culture dish. Cell adhesion and spreading were assayed in human ASM cells 72 h after transient transfection of scrambled and Bnip3 siRNA. A–C: cell adhesion and spreading are expressed as cell index and data are represented as real-time (A), peak (B), and integrated (area under the curve, AUC) (C) cell index (*P < 0.05, Bnip3 vs. scrambled siRNA; n = 3 ASM lines from 3 different donors). D: representative confocal images of rhodamine-phalloidin staining showing cell adhesion and spreading 1 and 3 h after seeding cells were seeded on culture dish. Bar = 100 μm. E: immunoblot showing efficiency of Bnip3 knockdown. C, control, scrambled siRNA; B, Bnip3 siRNA.