Fig. 6.

Bcl-2 adenovirus E1B 19 kDa-interacting protein 3 (Bnip3) downregulation in human airway smooth muscle (ASM) cells decreased paxillin, vinculin, and α-actinin protein levels. Expression of focal adhesion proteins and paxillin puncta in scrambled and Bnip3 siRNA-transfected human ASM cells. A: Western blot showing the protein levels of paxillin (pax), vinculin (vin), α-actinin (act), tensin 2 (ten), talin2 (tal), and Bnip3 using β-actin as loading control. B: quantified data with scrambled siRNA levels set at 1.0. *P < 0.05 significance relative to scrambled siRNA. C, scrambled siRNA; B, Bnip3 siRNA. C: representative confocal images of immunostaining for paxillin (green) in control (left panels) and Bnip3 knockdown (right panels; Bnip3 KD) human ASM cells on culture dish (top two panels; bar = 100 μm) and hydrogel (middle and bottom two panels; bar = 10 μm). Rhodamine (red) and DAPI (blue) were used as counterstaining for F-actin and nucleus, respectively.