Insulin-stimulated pAS160Ser588/AS160 at 3hPEX vs. SED controls in different fiber types from high-fat diet fed rats. The signal for pAS160Ser588 in type I fibers was insufficient to quantify; therefore, the data are not shown. *P < 0.05, indicates insulin significantly greater than no insulin within the same treatment group (SED or 3hPEX). †P < 0.05, indicates 3hPEX significantly different than SED within insulin treated fibers. Values are expressed as means ± SE; n = 7–10 per group. Representative blots and loading controls are included for each fiber type. MHC, myosin heavy chain; SED, sedentary; 3hPEX, 3 h postexercise.