Fig. 1.

Uptake of pyridoxine by pancreatic acinar cells (PAC) 266-6 as a function of time, and effect of incubation buffer pH. A: cells were incubated in Krebs-Ringer buffer (pH 7.4, 37°C) for different periods of time. B: initial rate (total) of pyridoxine uptake (30 min) at different incubation buffer pHs. Cells were maintained in DMEM growth medium, and before uptake experiments they were washed with Krebs-Ringer buffer. [³H]pyridoxine (7.5 nM) was added at onset of incubation. Data are means ± SE of 8–10 separate uptake determinations, respectively. When not shown, error bars are smaller than the symbols. *P < 0.05.