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Uptake of pyridoxine as a function of concentration in pancreatic acinar cells (PAC) 266-6. PAC 266-6 cells were maintained in DMEM growth media and before uptake experiments they were washed with Krebs-Ringer buffer. Cells were incubated at 37°C in Krebs-Ringer buffer at pH 7.4 in the presence of different concentrations of pyridoxine. Uptake by the saturable component was calculated as detailed in materials and methods. Data are means ± SE of 8 separate uptake determinations.
