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. 2020 Jan 13;11:232. doi: 10.1038/s41467-019-14141-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic illustration of the device for GUVs assembly: a SS mesh placed on the top center of a magnet. b Horizontal (top) and vertical (bottom) central section of the simulated magnetic field distribution across the microwells. The white dash box and the black dash circle respectively indicated the unit of the microwell array and the approximating unit for decentralized microwells. The black dash arrow indicated the preferential localization of GUVs around microwell wall. c Fluorescence images of the GUVs colonies with different extent of occupation of the microwells. The white dash circle indicated microwell wall. d Fluorescence image of GUV colony arrays formed in SS mesh with microwell diameter of 250 μm. e Top view and side view along the yellow dash section line of the GUVs colony taken by a laser confocal microscope. f A 3D image of GUVs colony obtained from serial sections of images in the Z-stacks taken by a laser confocal microscope. g Fluorescence images of GUVs colonies with different morphologies: from left to right, triangular, square, striped, and HIT-like assemblies. The dash triangle, rectangle, and line illustrated rough outline of GUVs colonies. h The schematic and simulated magnetic field distribution of SS mesh with densely packed microwells. The white dash box presented the unit of the microwell array. The black arrows indicated the corners. i Fluorescence images of the Chinese ancient coin-like round GUVs colonies with square holes formed using the SS mesh with densely packed microwells. The bottom is the enlarged image in the dash box of top image as indicated by the yellow dash arrow. The white dash box in bottom image indicated the square hole in GUVs colony. The scale bars are 100 μm.