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. 2020 Jan 13;10:168. doi: 10.1038/s41598-019-56867-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AP-MS analysis of Hho1-FZZ in three different physiological states of the Tetrahymena life cycle. Top: Western blotting analysis indicating recovery of the affinity purified Hho1-FZZ bait protein. The top panel represents the vegetative samples and the bottom panel shows the recovery of the bait from conjugation samples. The blots were probed with anti-FLAG antibody. No signal was detected in samples from the untagged control cells. Bottom: Network illustration of the Hho1-FZZ co-purifying proteins at statistical threshold of FDR ≤ 1% in three different stages of the Tetrahymena life cycle; vegetative growth, starvation (24 hours) and conjugation (6 hours post mixing of the cells). Node color key is provided.