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. 2020 Jan 13;11:241. doi: 10.1038/s41467-019-14145-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Pulldown assay with CsgG and different parts of CsgF (C-terminal MBP tag). * Indicates CsgF degradation. Source data are provided as a Source Data file. b Pulldown assay with CsgB(C-terminal MBP tag) and different parts of CsgF (N-terminal MBP tag). * Indicates CsgB degradation. Source data are provided as a Source Data file. c Congo red cell-association assay for different parts of CsgF. Source data are provided as a Source Data file. d Pulldown assay of CsgF(N-terminal MBP tag), CsgB(N-terminal MBP tag) and CsgA(C-terminal MBP tag). * Indicates CsgA degradation. Source data are provided as a Source Data file. e Overall assembly model of curli biogenesis system. OM outer membrane.