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. 2020 Jan 13;11:241. doi: 10.1038/s41467-019-14145-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Size-exclusion chromatogram of the purified CsgFG and CsgA_N22-PhoA complex. b SDS-PAGE analysis and Coomassie blue staining of the purified CsgFG and CsgA_N22-PhoA complex from SEC. * Indicates PhoA degradation. Source data are provided as a Source Data file. c Congo red secretion assay for CsgA_N22 truncation mutant. Source data are provided as a Source Data file. d Side (top) and top (bottom) view of the CsgFG-CsgA_N22 complex structure. CsgG, CsgF, and CsgA_N22 are in cyan, orange and red, respectively. OM outer membrane.