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. 2020 Jan 13;10:197. doi: 10.1038/s41598-019-57064-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dose-dependence of anti-inflammatory effect of FL peptide. (A) Inflammatory cells that infiltrated to the bronchus were measured in BALF using HEMAVET 950FS. (B) H&E staining of lung tissues was performed to visualize cell infiltration. The a indicates the airway, and red arrows indicate inflammatory infiltrates. (C) IL-5 level in BALF was measured using ELISA. (D) OVA-specific IgE in serum was measured using ELISA. (E) Lung tissue was homogenized and immunoblotted with phospho IκBα and beta actin antibodies. (F) BALF was concentrated and immunoblotted for TCTP. Each lane represents biological replicate indicated by the number. PC: positive control (n = 3), FL 1: FL 1 mg/kg (n = 3), FL 20: FL 20 mg/kg (n = 3), WBC: white blood cells, NE: neutrophils, LY: lymphocytes, MO: monocytes, EO: eosinophils, BA: basophils. Values represent mean ± SEM, *p < 0.05, **p < 0.01; compared to PC.